| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171523 |
| Chromosome: | chromosome_12 |
| Location: | 4126033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g518050 | FAP261 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGCCATTAGGGGGGCAGTGGGGAGGGTG |
| Internal bar code: | TCTAGCTTATGGGGTTTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.6278 |
| LEAP-Seq n confirming: | 6959 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGATCTACACGCTGGG |
| Suggested primer 2: | AACATTTTCGGAGTGATGGC |