Insertion junction: LMJ.RY0402.171530_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.171530
Chromosome:chromosome 3
Location:8946619
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g208833 (1 of 1) K10896 - fanconi anemia group M protein (FANCM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTGCGGGCTGCTTACGTTGTAGGTGC
Internal bar code:CACTGTCCCGTCCTCTTAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:816
LEAP-Seq percent confirming:99.8478
LEAP-Seq n confirming:656
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCAGTAAGCTCATTCC
Suggested primer 2:AACAACAGCCACAGCAACAC