| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171560 |
| Chromosome: | chromosome 9 |
| Location: | 1775860 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396350 | MITC3 | (1 of 2) PTHR24089//PTHR24089:SF51 - FAMILY NOT NAMED // MITOFERRIN-1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACTACCAAGGGCATCCCCTTGCAGCT |
| Internal bar code: | GCACGGAGCCGGGCGTGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 99.855 |
| LEAP-Seq n confirming: | 2066 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGGCGAAATCTCGAAC |
| Suggested primer 2: | CCATGTTTGTATGTGCCTGC |