| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171579 |
| Chromosome: | chromosome 14 |
| Location: | 1051978 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614600 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAAGGAGCTTGAGGATGTGCGTGAGG |
| Internal bar code: | AAGTTTGAGGGGCACGTTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.4296 |
| LEAP-Seq n confirming: | 6275 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGAGTCAGTTGCACAGG |
| Suggested primer 2: | CCATTTCCACAATGAAACCC |