| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171580 |
| Chromosome: | chromosome 14 |
| Location: | 597739 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611950 | PPR-superfamily protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCCAAACCAGACAGTCAGGCCCCGCA |
| Internal bar code: | CGTACCGTGTTCAACATGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.2299 |
| LEAP-Seq n confirming: | 8376 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGTGAAATTCAGCGCAC |
| Suggested primer 2: | AGCTTCAGGACCAGACGAAA |