| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171593 |
| Chromosome: | chromosome 17 |
| Location: | 3856660 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728200 | (1 of 1) K11664 - vacuolar protein sorting-associated protein 72 (VPS72, TCFL1, YL1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGGGCGAAAATGGGGCGAGACCTCCC |
| Internal bar code: | CATTCCCCCGTAGTCCGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 98.6508 |
| LEAP-Seq n confirming: | 1243 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGATGAGAGGGAAGGGT |
| Suggested primer 2: | GACCTTCGCAACCGTACATT |