| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171614 |
| Chromosome: | chromosome 11 |
| Location: | 1849938 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467900 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCCACTGTTGAATGGTGGAAGGGTGGG |
| Internal bar code: | GCAGGTAACAGGAGTTAAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 92.1986 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCCTCACACCATACGC |
| Suggested primer 2: | AAGTGCGCATTCGTTTCTTT |