| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171622 |
| Chromosome: | chromosome 12 |
| Location: | 1370693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488350 | (1 of 2) K00464 - all-trans-8'-apo-beta-carotenal 15,15'-oxygenase (diox1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGCTAACGTTGCGGGCCCCTAATCCA |
| Internal bar code: | CCGCTGGTAGCCTCCATTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 99.6293 |
| LEAP-Seq n confirming: | 1075 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCAGATACCTTTCACAG |
| Suggested primer 2: | GCAACCAACTGAGTGCAAGA |