| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171650 |
| Chromosome: | chromosome 17 |
| Location: | 4664327 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATCAAAAGCAAGCAGCATGTCAAGAAC |
| Internal bar code: | GATCACGCTTGATGGCGGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 96.8121 |
| LEAP-Seq n confirming: | 1731 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGCGCGAATTTTGACA |
| Suggested primer 2: | AGCTTCATGTTCGCGCTTAT |