| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171678 |
| Chromosome: | chromosome_16 |
| Location: | 5634871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g679550 | FAP277 | Flagellar Associated Protein, protease inhibitor-like | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCCAAAAAGGTCGAACGACTCACCCGCG |
| Internal bar code: | GCTGTCTGTATTAACTGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.8736 |
| LEAP-Seq n confirming: | 3160 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACTCACTGGTCGGTTT |
| Suggested primer 2: | ATGGTAAAGCCGTTGGACAG |