| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.171679 |
| Chromosome: | chromosome 12 |
| Location: | 697334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494850 | ADK3 | Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF72 - NUCLEOTIDE KINASE // ADENYLATE KINASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCACGCTTTAGAGGGTTGCACCGGTGC |
| Internal bar code: | GGGCGCCGGTGCCAAGTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.7467 |
| LEAP-Seq n confirming: | 11815 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGATAACATCCAGGCG |
| Suggested primer 2: | GGCATTCTAGCTCAAGCCAC |