| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171685 |
| Chromosome: | chromosome 14 |
| Location: | 1685691 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619300 | CYN16 | Cyclophilin 16; (1 of 60) 5.2.1.8 - Peptidylprolyl isomerase / Rotamase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGATATTGACATTGGTGATGCAGCACGT |
| Internal bar code: | TCCGTTATGGGCAAGATCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.8897 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGGTGCGATATACGTG |
| Suggested primer 2: | ACCGTTATGCGTAGGATTGC |