| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171699 |
| Chromosome: | chromosome 2 |
| Location: | 5429535 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCGGGTCCCGTGCCCCTCAGCGCGGGC |
| Internal bar code: | GCTGGGCATCTTGTGAGTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 97.6831 |
| LEAP-Seq n confirming: | 2867 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACACAGGATGTGCAAGGC |
| Suggested primer 2: | AGGTGGGACTGAAACTGGTG |