| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171771 |
| Chromosome: | chromosome 2 |
| Location: | 4149407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098100 | (1 of 1) PTHR14134//PTHR14134:SF3 - E3 UBIQUITIN-PROTEIN LIGASE RAD18 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTTCCACACCAGGCTTGGGGAGCGATG |
| Internal bar code: | CCAGTCACGAATGGACGCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 97.5372 |
| LEAP-Seq n confirming: | 1703 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACTTCCCACAGCAATCC |
| Suggested primer 2: | TATCGCATTGCCCTATCCTC |