| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171780 |
| Chromosome: | chromosome 10 |
| Location: | 5802366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTCTCGGAGCTCGCGCGCGCGCCGAT |
| Internal bar code: | CATATAACCAAGGGGGCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 99.6855 |
| LEAP-Seq n confirming: | 634 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACAATGTCATGCTTCTCG |
| Suggested primer 2: | AGCCATAGCTCTGCGATTGT |