| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171848 |
| Chromosome: | chromosome_10 |
| Location: | 90012 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g418250 | BLD10 | Basal body protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGAGGCGCTGCGTGAGGAGCTGCGGGCGG |
| Internal bar code: | CCTTGGTCGACTCCCGGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGAAGACATGGAGAAGC |
| Suggested primer 2: | CTTAGCGTACTAGGTGGCCG |