| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171865 |
| Chromosome: | chromosome 12 |
| Location: | 4429657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521150 | CGL19 | (1 of 1) PTHR31089//PTHR31089:SF1 - FAMILY NOT NAMED // CYCLIC DOF FACTOR 4-RELATED; DOF type transcription factor | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTCGTTGCGATCGCAGGCTGTGGTGGC |
| Internal bar code: | TTATGTTCCGATTTACAACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1165 |
| LEAP-Seq percent confirming: | 98.6711 |
| LEAP-Seq n confirming: | 891 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTTGCCTTGCAGTAA |
| Suggested primer 2: | ACGAGTGCTGATGGGGTTAC |