| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171877 |
| Chromosome: | chromosome 10 |
| Location: | 4349535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451250 | (1 of 3) PTHR13620:SF0 - EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAAGCTAGCAACGCCAGATGATGAAGA |
| Internal bar code: | TTTCAAGTTCCGTTGTTCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 793 |
| LEAP-Seq percent confirming: | 99.3122 |
| LEAP-Seq n confirming: | 2888 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCTGCTGAAAGGCACAA |
| Suggested primer 2: | GCCTGTCGTGATCTACCCAT |