| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171920 |
| Chromosome: | chromosome 5 |
| Location: | 3193054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239750 | ARS13 | (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACGGCGGTTTCAAAAAGTTCTCGTAC |
| Internal bar code: | CCTGGGCGTTCCCGGTAGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 86.747 |
| LEAP-Seq n confirming: | 1728 |
| LEAP-Seq n nonconfirming: | 264 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAACCCATCATCATCAG |
| Suggested primer 2: | CTGCACGCCATCAAGTTCTA |