Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.171920 |
Chromosome: | chromosome 5 |
Location: | 3193065 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g239750 | ARS13 | (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGTCCATGGCTTGTTCCAGGTTGGTCA |
Internal bar code: | TCCGGGCGTGGAATTCACTAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 638 |
LEAP-Seq percent confirming: | 98.2719 |
LEAP-Seq n confirming: | 853 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTCAACCCATCATCATCAG |
Suggested primer 2: | CTGCACGCCATCAAGTTCTA |