Insertion junction: LMJ.RY0402.171920_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.171920
Chromosome:chromosome 5
Location:3193065
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g239750 ARS13 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACGTCCATGGCTTGTTCCAGGTTGGTCA
Internal bar code:TCCGGGCGTGGAATTCACTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:638
LEAP-Seq percent confirming:98.2719
LEAP-Seq n confirming:853
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAACCCATCATCATCAG
Suggested primer 2:CTGCACGCCATCAAGTTCTA