| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.171972 |
| Chromosome: | chromosome 17 |
| Location: | 2439348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715700 | VTC3,PPP44,PP2C5 | (1 of 1) PF00481//PF07714 - Protein phosphatase 2C (PP2C) // Protein tyrosine kinase (Pkinase_Tyr); protein phosphatase 2C-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCCCCGCAGGTCGTCTAGGGTTGCAA |
| Internal bar code: | AGTGGTCGTTTCTTCCATTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.5033 |
| LEAP-Seq n confirming: | 601 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGGTATGAAGGAGCGGG |
| Suggested primer 2: | CTGTAGGATGACGGTTCGGT |