| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.171974 |
| Chromosome: | chromosome 11 |
| Location: | 1794183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467500 | (1 of 1) PF05664 - Plant family of unknown function (DUF810) (DUF810) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTTTTGCTTGCGTACCGTACTTGGTGC |
| Internal bar code: | ATGCAACCTCTCCGCGACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 99.5577 |
| LEAP-Seq n confirming: | 2926 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACAGCCTGTCTACCAC |
| Suggested primer 2: | ACGGCTTCATATTTTCACGG |