| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.171994 |
| Chromosome: | chromosome 3 |
| Location: | 5342566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184100 | (1 of 2) PF08797 - HIRAN domain (HIRAN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGGACTCCATTCGGCTGGCAGGCGTCA |
| Internal bar code: | CGTCGTGTGCGTTGCGTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.896 |
| LEAP-Seq n confirming: | 1922 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCTGTCCAGCACTATG |
| Suggested primer 2: | TCATGGCAAGTTCAGTCTGC |