Insertion junction: LMJ.RY0402.171997_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.171997
Chromosome:chromosome 9
Location:2196985
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g393050 PRM6,PRM, PRMT6 (1 of 1) PTHR11006//PTHR11006:SF4 - PROTEIN ARGININE N-METHYLTRANSFERASE // PROTEIN ARGININE N-METHYLTRANSFERASE 7; Protein-/Histone-arginine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGTAAAATACGCACACACACACACAC
Internal bar code:AGCCGCGAGCAGAAGTGCCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:866
LEAP-Seq percent confirming:41.5304
LEAP-Seq n confirming:4369
LEAP-Seq n nonconfirming:6151
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GACCACGCTTTGGTTTCATT
Suggested primer 2:GTCTGTCTCGTTGTGTGCGT