| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172019 |
| Chromosome: | chromosome 2 |
| Location: | 5421132 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107400 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCTCTTCCTAAAACTCAGTTGGTTGG |
| Internal bar code: | TGACCAGAGGCGGAAGGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 99.9564 |
| LEAP-Seq n confirming: | 2292 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAAGGATGGATGCTTGGA |
| Suggested primer 2: | ATCAGCAACCCCGTACAGAC |