| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172020 |
| Chromosome: | chromosome 9 |
| Location: | 3219684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387615 | (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCTTCGGAAGGCGAGCATGTTGAGAG |
| Internal bar code: | GTCCTCTGCTTAGTACGCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGCACTTATGTGAAGGG |
| Suggested primer 2: | TGGACATGAGTGGAGCTCAG |