| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172054 |
| Chromosome: | chromosome 5 |
| Location: | 855304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247851 | CEP2 | Cysteine endopeptidase; (1 of 2) 3.4.22.14 - Actinidain / Actinidin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAACCCTACATGCCGGCACCGTGCTGA |
| Internal bar code: | GGACAGCACCCCGTGATTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 99.0937 |
| LEAP-Seq n confirming: | 328 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAACGAGATGTGTGTGG |
| Suggested primer 2: | CTGTGGTTGGACTCTACGCA |