| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172093 |
| Chromosome: | chromosome_17 |
| Location: | 1145028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g704300 | FAP47 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCTCGAGTGGCGGCGGCGGCGTGATCCGC |
| Internal bar code: | GTCCTATGCAGCAGGTGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 97.9807 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTTGGCAAGGTGTCCAT |
| Suggested primer 2: | ATCTGCATCATCACGCTCTG |