| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172124 |
| Chromosome: | chromosome 14 |
| Location: | 1982711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCCATGTGGTCGCGACGGTGGAAGCTG |
| Internal bar code: | GGTGAAGGGTTCGCTGATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 99.8339 |
| LEAP-Seq n confirming: | 2404 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGAGATTTGGAGGATGG |
| Suggested primer 2: | GAGTACGTCAAGTGCGACGA |