| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172159 |
| Chromosome: | chromosome 4 |
| Location: | 4021966 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g232402 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGACAGACCGACTCACATTTCAGCCC |
| Internal bar code: | GCGTGAGCAACAGAACGCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 98.8201 |
| LEAP-Seq n confirming: | 7454 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTATCGGTGTGCTGCTG |
| Suggested primer 2: | ATTCAGTGGGTCGTAGTCGG |