| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172184 |
| Chromosome: | chromosome 9 |
| Location: | 3245482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387763 | (1 of 1) 1.1.1.94 - Glycerol-3-phosphate dehydrogenase (NAD(P)(+)) / L-glycerol-3-phosphate:NAD(P) oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGATACACACCCGCACACGCACCCCTG |
| Internal bar code: | GATCTTGTATTCGGCATGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.5319 |
| LEAP-Seq n confirming: | 13183 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | AACGGAGTGAGTGGACCAAG |