| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172203 |
| Chromosome: | chromosome 17 |
| Location: | 4276191 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730700 | (1 of 1) PTHR11732//PTHR11732:SF2 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGTCGCTGCGCGGCCGCGCCGACGTC |
| Internal bar code: | CCCGGGCCTAGGCGGGCGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 92.0705 |
| LEAP-Seq n confirming: | 1672 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGAGGGTCTTGAACAGC |
| Suggested primer 2: | CCTTACCGTATGCCCATGTC |