| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172224 |
| Chromosome: | chromosome 2 |
| Location: | 5542958 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108601 | (1 of 1) 2.7.10.2//2.7.11.25//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Mitogen-activated protein kinase kinase kinase / MLTK // Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGAGGTCCGGATCTCCACGTTTCAGC |
| Internal bar code: | GCCGACGCGCTATCGCAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 99.6557 |
| LEAP-Seq n confirming: | 2026 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAATCTGGTGTCTACGC |
| Suggested primer 2: | GATGTGGCGTGTTAGGGAGT |