| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172236 |
| Chromosome: | chromosome 2 |
| Location: | 3844637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095750 | ZEP1,ZXE1 | (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATCCAGTTTACGGCATTCGCTCTCGC |
| Internal bar code: | CCAGTCCTGGGCCCCGATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 99.3846 |
| LEAP-Seq n confirming: | 323 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTGGCACAATGACACG |
| Suggested primer 2: | TCCTGCCTGTCTCATTCCTT |