| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172262 |
| Chromosome: | chromosome 13 |
| Location: | 4101857 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591501 | PUS2 | (1 of 2) PTHR11142:SF0 - TRNA PSEUDOURIDINE SYNTHASE-LIKE 1; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCCCCAGGCCTTGCCTACGGTGACTT |
| Internal bar code: | CGTATGGTTGGGCGGAGTAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.7037 |
| LEAP-Seq n confirming: | 1346 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGACGGCACCGACTACT |
| Suggested primer 2: | AAACCAACACCAGAACCAGC |