| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172270 |
| Chromosome: | chromosome 7 |
| Location: | 1891364 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325739 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGTATGGTGCGCAAGGAGCAAGCGTTG |
| Internal bar code: | CTAGTGATCACGGACCCGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 99.6568 |
| LEAP-Seq n confirming: | 6388 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAGCCACCAGTTGTTCCC |
| Suggested primer 2: | ACTTATCCAGGCCACGTCAC |