| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172290 |
| Chromosome: | chromosome 3 |
| Location: | 4077842 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172750 | (1 of 1) IPR002893//IPR012677 - Zinc finger, MYND-type // Nucleotide-binding alpha-beta plait domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACGAGCACTGGTCCATTTGGTCAGTG |
| Internal bar code: | ATATCGGACTAGTTACTGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 97.9381 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAAAGTCCGAGCAAGTCG |
| Suggested primer 2: | ATAGCCCAACTCACCCACTG |