| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172326 |
| Chromosome: | chromosome 12 |
| Location: | 6071447 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535500 | (1 of 103) IPR000504 - RNA recognition motif domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTTCGCACCAAGAATACCGCAATCAAG |
| Internal bar code: | CGAGACGTGTCTGACTGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.7559 |
| LEAP-Seq n confirming: | 4087 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTCGTCACTGTCCACCA |
| Suggested primer 2: | TTGCAAGTGGCTTGTGGTAG |