Insertion junction: LMJ.RY0402.172333_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.172333
Chromosome:chromosome 4
Location:190567
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217050 (1 of 173) IPR013083 - Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCAGTCCGCGGTGGAGGCGCGCGAGGCA
Internal bar code:GCCCAGAATTCCGCGGGTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:60
LEAP-Seq percent confirming:31.7952
LEAP-Seq n confirming:565
LEAP-Seq n nonconfirming:1212
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACAGCAACAGCAGCAAC
Suggested primer 2:CTTCTCCTTCTCCGCCTTCT