| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172369 |
| Chromosome: | chromosome 4 |
| Location: | 138300 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216826 | 3'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGATCCGGTGATTGCAGGTAGACAGCT |
| Internal bar code: | GGCACGGGCCGGCGTTCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 46.8668 |
| LEAP-Seq n confirming: | 359 |
| LEAP-Seq n nonconfirming: | 407 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTGACTAGGGCGAGAG |
| Suggested primer 2: | GGACAGCATCAGAGCATTCA |