| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172374 |
| Chromosome: | chromosome 16 |
| Location: | 2063801 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657300 | CPI1 | Cyclopropyl isomerase-like protein; (1 of 1) 5.5.1.9 - Cycloeucalenol cycloisomerase / Cycloeucalenol--obtusifoliol isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAACATGGTTGTGCCATGGACATAAGG |
| Internal bar code: | ACGTACACCGTTGGGACAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 98.3425 |
| LEAP-Seq n confirming: | 356 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCATCCCAAGTTAGTGTT |
| Suggested primer 2: | AAGATGTACACCGTGGGCTC |