| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172392 |
| Chromosome: | chromosome 12 |
| Location: | 1514025 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487150 | PGM20 | (1 of 2) PTHR23029//PTHR23029:SF24 - PHOSPHOGLYCERATE MUTASE // SUBFAMILY NOT NAMED; Putative phosphoglycerate mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGATCGGGAGAGCGCCATCCGAGCTGAC |
| Internal bar code: | GCCTCGCATTATCAAGCATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 89.7161 |
| LEAP-Seq n confirming: | 1675 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCTTGAGCTGGGATGC |
| Suggested primer 2: | TTAAAACTTCACGAGCGCCT |