| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172399 |
| Chromosome: | chromosome 1 |
| Location: | 7247914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052250 | TRXx,TRX1 | Thioredoxin x | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGTGTCGTCACCGCAGCGTGTCACAG |
| Internal bar code: | AGTTACGAGCGCGATAAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 99.7516 |
| LEAP-Seq n confirming: | 8434 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCAAGCCAACATCAACA |
| Suggested primer 2: | ACTGGCGTAGGTGTTTGACC |