| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172414 |
| Chromosome: | chromosome 14 |
| Location: | 2647586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626300 | (1 of 10) PF05347 - Complex 1 protein (LYR family) (Complex1_LYR) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACAGAAGCTCAGCGGTCAGCCTTGATGA |
| Internal bar code: | TGAGTGCCTTTGCACTTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 98.6207 |
| LEAP-Seq n confirming: | 4147 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAATTTGTCGCGTTGTCTC |
| Suggested primer 2: | AGCCTGTGAGCTTTGACGAT |