| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172417 |
| Chromosome: | chromosome 12 |
| Location: | 8681013 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546500 | TEF22 | Predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTTCCGTCATTTTAGCAGTGTCACCGC |
| Internal bar code: | AATGTGGCCAATAGCAGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 627 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATACCACGTCCAGCAC |
| Suggested primer 2: | CAGCTGCCTAGCACACCATA |