| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172431 |
| Chromosome: | chromosome 3 |
| Location: | 9085606 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210961 | (1 of 1) PTHR10174:SF127 - PHOSPHATIDYLINOSITOL TRANSFER PROTEIN PDR16-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGCTTTCCACTCGAGCAAATTTCCCTT |
| Internal bar code: | GCGCTTTTTGTTGACAATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 98.4664 |
| LEAP-Seq n confirming: | 2504 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAAACGCGCACTATTTA |
| Suggested primer 2: | AAGTACACCGATCCACGCTC |