Insertion junction: LMJ.RY0402.172462_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.172462
Chromosome:chromosome 6
Location:8875378
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g310850 (1 of 12) IPR003439//IPR003593//IPR027417 - ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGACCTGGAGGCGGCGCTCAGGTGCGGG
Internal bar code:TCTTGTAGGCTGCTGGTTCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:99.0566
LEAP-Seq n confirming:1260
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCTAACCTGCACGAACTT
Suggested primer 2:GTCCTGTGGTCCTCATCGTC