| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172466 |
| Chromosome: | chromosome 10 |
| Location: | 2883814 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439950 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATACCCAATGGCTGCTTGCGCATCTTCC |
| Internal bar code: | TGGCCTTCGGCCCTAAGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.6273 |
| LEAP-Seq n confirming: | 2673 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCGCAGTAGATGTCAAG |
| Suggested primer 2: | ACATGTTCCCTGCATAAGGC |