| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172492 |
| Chromosome: | chromosome 2 |
| Location: | 431068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076200 | (1 of 1) 3.4.11.2 - Membrane alanyl aminopeptidase / Peptidase E | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCCGGCGGGGCTGGGCGGCAGGCTC |
| Internal bar code: | ATTGAGACAATAGAGTAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 84.9594 |
| LEAP-Seq n confirming: | 1463 |
| LEAP-Seq n nonconfirming: | 259 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGGAGTGAAGGTGATG |
| Suggested primer 2: | AAGAGCCACCCCTTGTTTTT |