Insertion junction: LMJ.RY0402.172493_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.172493
Chromosome:chromosome 11
Location:332018
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467568 (1 of 1) K06276 - 3-phosphoinositide dependent protein kinase-1 (PDPK1) 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGCGTGAGCGCGCGGGTGGTTGAACG
Internal bar code:CCCCATCGTGTCGAATCACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1009
LEAP-Seq percent confirming:97.026
LEAP-Seq n confirming:261
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCTTGAGGTTACGCTTGA
Suggested primer 2:GGGCGCTCACATGAATCTAT