Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.172493 |
Chromosome: | chromosome 11 |
Location: | 332018 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467568 | (1 of 1) K06276 - 3-phosphoinositide dependent protein kinase-1 (PDPK1) | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGCGTGAGCGCGCGGGTGGTTGAACG |
Internal bar code: | CCCCATCGTGTCGAATCACATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1009 |
LEAP-Seq percent confirming: | 97.026 |
LEAP-Seq n confirming: | 261 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCTTGAGGTTACGCTTGA |
Suggested primer 2: | GGGCGCTCACATGAATCTAT |